Optimization of supercritical fluid extraction of geniposidic acid from plantain seeds using response surface methodology

摘要

The process parameters of supercritical CO2 (SCCO2) plus modifer for the extraction of geniposidic acid from plantain seeds were studied using a Box–Behnken design. The effects of independent variables, that is, ethanol concentration (0–70%, ethanol:water, v/v), extraction pressure (10–30 MPa), and temperature (50–80°C) on the yield of geniposidic acid were evaluated. Results indicated that the data could be well fitted to a second-order polynomial model. The effects of ethanol concentration and temperature, as well as the interaction between ethanol concentration and temperature were significant (p 0.05). The yield (8.9 mg/g) of modified SCCO2 extraction at optimal conditions was compared with that obtained by Soxhlet extraction or ultrasound assisted extraction.