Two dimensional gel electrophoresis analyses of human plasma proteins. Association of retinol binding protein and transthyretin expression with breast cancer

摘要

The identification of markers for either early diagnosis, treatment response or for survival of breast cancer is of critical importance. The plasma carries an archive of important histological information whose determination may help to improve early disease detection. Using two dimensional gel electrophoresis and protein sequencing we investigated the changes in protein expression profiles derived from analysis of plasma from healthy Tunisian women and patients with breast carcinoma. We have found an association between retinol binding protein, transthyretin expression and breast cancer. The levels of acute phase proteins known to accompany both acute and chronic inflammatory disorders comprising haptoglobin, serum amyloid P, apolipoprotein A1, α1-antitrypsin and α1-acidic glycoprotein were also intimately associated with this neoplastic disease.