In vitro Effect on the Antioxidative Properties of Crude Extract of C. album in Presence of the Organophosphate, Acephate

摘要

Acephate, an organophosphorus pesticide is a potent free radical generator. The aim of this study was to evaluate the role of plant phenolics in Chenopodium album against acephate toxicity in vitro, predominantly acting as a free radical scavenger. Experiments based on DPPH radical scavenging activity and reducing power of plant extract both in the presence and absence of acephate was analyzed using concentrations of 1, 2.5 and 5 μg mL-1 of polyphenolic compounds and 3 mg mL-1 of acepahte which showed that pesticides significantly reduced the activity of the plant extract and a higher concentration was required to quench the ABTS+. radicals. No significant change was observed in case of lipid peroxidation in presence of acephate. Thus, from this study, it is evident that pesticides act as a serious free radical source whose effect can be reduced, if not totally but to a certain extent by green leafy vegetables.