APPLICATION OF A ONE-STEP REAL TIME RT-PCR PROTOCOL FOR NOROVIRUS DETECTION IN SHELLFISH HARVERSTED AND COMMERCIALIZED IN CAMPANIA REGION

摘要

Shellfish are recognized as a potential vehicle of viral diseases. However, according to European Regulations, only bacteriological parameters (Salmonella and E. coli) are used to establish their microbiological quality. Because of the lack of a standard reference method for the detection of viruses, data on the incidence of enteric viruses in European production areas are still limited. Aim of the present study was the evaluation of the Norovirus (NoV) contamination in shellfish from different harvesting areas (both class A and B) and from registered and not- registered retails of the Campania region (Tyrrhenian Sea, Italy). Seventy shellfish (Mytilus galloprovincialis) were collected from 15 harvesting areas, one authorized and two not authorized retails and were examined for virus presence (NoV genogroups I and II) by Real Time PCR. Ten harvesting areas (66%) showed presence of viral contamination, with three of them belonging to A areas and seven to B areas. The shellfish collected from the registered retail tested positive for Norovirus presence, whereas the shellfish collected from not-authorized retails didn’t show viral contamination. Twenty-nine samples were positive for genogroup II, ten of which tested positive also for NoV genogroup I.