Effects of in ovo Injection of Vitamins B6 and B12 in Fertile Eggs Subjected to Ethanol Stress on Hatching Traits, Performance and Visceral Organs of Broiler Chicks Reared under Cold Stress Condition

摘要

Two subsequent experiments were conducted to evaluate the effects of in ovo injection of vitamin B6 and B12 in fertile eggs subjected to ethanol (EtOH) stress on hatching traits (first), performance and visceral organs of broiler chicks under cold stress (second). A number of 510 fertile eggs were incubated. A number of 180 eggs were considered as controls (three subgroups as: not-injected, eggshell with a hole and distilled water-injected). A number of 110 eggs were injected with 25 μL of a 1:1 (v/v) mixture of EtOH 47.5% + distilled water. Eggs in two other groups were injected with 25 μL of a 1:1 (v/v) mixture of EtOH 47.5% + 100 μL of B6 (n=110), 25 μL of a 1:1 (v/v) mixture of 47.5% EtOH + 1000 μL of B12 (n=110). A number of 240 one-day chicks, allocated to second experiment. Hatched chicks were divided into 4 treatments. Temperature was maintained 12 ?C from 28 to 42 days of age. Hatchability percent (P 0.05). In ovo injection of vitamins B6 and B12 alleviated EtOH-induced oxidative stress in chickens embryos. No significant difference was observed in the performance of the hatched birds in the cold conditions temperature (P>0.05).