PREVENTION OF OBESITY AND DEVELOPMENT OF METABOLIC SYNDROME BY MANGOSTEEN (GARCINIA MANGOSTANA L.) PERICARP ETHANOLIC EXTRACT IN MALE WISTAR RATS FED WITH HIGH-FAT DIET

摘要

Objective: This study was conducted to evaluate the mangosteen ( Garcinia mangostana ) pericarp ethanolic extract (MPEE) properties in preventing obesity as well as its associated metabolic syndrome by analyzing the liver histology and measuring resistin, leptin, and adiponectin in the rats fed high-fat diet. Methods: The experimental study with male Wistar rats was conducted for 9 w, with rats divided into 5 treatment groups which were normal (standard diet), control (high-fat diet), dose 1 (high-fat diet, MPEE 200 mg/kg b.w.), dose 2 (high-fat diet, MPEE 500 mg/kg b.w.), and orlistat (high-fat diet, orlistat 21.6 mg/kg b.w.) groups. The fat and liver index were calculated at the last day of the experiment. The liver was collected for the histology analysis while the serum was collected for quantification of resistin, leptin, and adiponectin level by enzyme-linked immunosorbent assay (ELISA) method. Results: MPEE significantly reduced the liver index and total fat index compared to the control group. The rats treated with MPEE have the better liver condition than untreated rats, showed by lower histopathological score and lipid droplets, as well as higher percentage of functional cytoplasm. The MPEE also able to reduce the resistin and leptin level in high-fat diet rats, and slightly increase the adiponectin level. MPEE have better activities in all parameter compared to commercially available anti-obesity drug orlistat. Conclusion: The mangosteen pericarp ethanolic extract (MPEE) has high potential as anti-obesity drugs, clinical and toxicology studies should be pursued for further application to human. Keywords : Garcinia mangostana , Obesity, Fatty liver, Insulin resistance, Resistin, Adiponectin, Leptin