EVALUATION OF INVITRO ANTI-OXIDANT AND ANTICANCER ACTIVITY OF CORIANDRUM SATIVUM AGAINST HUMAN COLON CANCER HT- 29 CELL LINES

摘要

Objective: The main aim of the study was to screen the ethanolic extract of Coriandrum Sativum leaves for its invitro anti-oxidant and anticancer activity and find its efficacy against H-29 Cell lines. Methods: Ethanolic extract of Coriandrum Sativum were prepared and assayed for the presence of phytochemicals. In vitro antioxidant assay were performed by DPPH radical assay and FRAP assay. The effect of ethanolic extracts on HT-29 cancer cell lines were evaluated by MTT colorimetric assay. Results: The preliminary phytochemical screening of ethanolic extract showed the presence of significant secondary metabolites. The efficacy of Coriandrum Sativum against HT-29 cell lines showed that the incubation of cancer cells reduced the viability of HT-29 cell lines and the dead cells were significantly increased with high extract concentration. Hence Ethanolic extract of Coriandrum Sativum exhibited high cytotoxicity. Conclusion: Even at very low concentration Coriandrum Sativum showed high efficacy. In conclusion Coriandrum Sativum possess significant antioxidant activity and anticancer activity