DEVELOPMENT AND VALIDATION OF A SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LEVOFLOXACIN BOTH IN BULK AND PHARMACEUTICAL FORMULATION

摘要

The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of levofloxacin in bulk and pharmaceutical formulation. The solvent used throughout the experiment was 0.1N NaOH. Absorption maximum (λ_max) of the drug was found to be 288 nm. The quantitative determination of the drug was carried out at 288 nm and Beer’s law was obeyed in the range of 2-12μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.0704x + 0.0034 with correlation coefficient R2of 0.999. The recovery values for levofloxacin ranged from 99.92% – 100.33%. The relative standard deviation of six replicates of assay was less than 2%.The percent relative standard deviation (RSD %) of interday precision range was 0.469 – 0.925 % and intraday precision range was 0.685 – 0.713%. The limit of detection and limit of quantification was 0.087μg/mL and 0.164μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.136 – 0.213%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.