The Protective Effects of Thymus vulgaris Aqueous Extract against Methotrexate-Induced Hepatic Toxicity in Rabbits”

摘要

The present study was aimed with Cheese processing effluent sample were collected from Sri Ganapathy Dairy farms, RS Puram, Coimbatore District. These samples were subjected to analyze the physico-chemical parameters include pH, Salinity, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Total Suspended Solids (TSS), Total Dissolved Solids (TDS), Total Solids (TS) before and after treatment. Estimation of Chloride, Phosphate and Calcium content were assessed by titrimetric and turbidity method respectively. The cheese processing effluent was serially diluted and transferred (10-4 to 10-6 dilution) to the nutrient agar medium and incubated. After incubation two bacterial species namely Lactobacillus bulgaricus and Lactobacillus fermentum were isolated and identified using morphological and biochemical characters. Among these, Lactobacillus bulgaricus were effectively degrade the effluent sample compared with Lactobacillus fermentum. The effect of treated effluent (Lactobacillus bulgaricus and Lactobacillus fermentum) on growth of Vigna mungo was determined by pot culture experiment. After 28th day of seedlings, the better response observed in morphological and phytochemical characteristics of Vigna mungo were recorded in T2 than other treatments.