Construction and Immunogenicity of Recombinant Phage T7 Expressing Capsid of Porcine Circovirus Type 2

摘要

Porcine Circovirus type 2 (PCV2) is the causative agent of porcine circovirus disease (PCVD) that is regarded as one of the most important diseases endangering pig industry development. Immunization is one of the most effective ways to prevention and control PCV2 infection. Here, gene encoding capsid protein of a PCV2 from a farm of Chongqing was optimized, synthesized and inserted into the coat protein 10B gene of T7 phage to construct recombinant phage T7-Cap. Capsid protein expressed on the surface of phage T7-Cap was tested using western blot. Anti-PCV2 antibody in the serum was detected after the swine inoculated with T7-Cap. The virus clearance from the serum was assayed by PCR after T-Cap vaccinated pigs were challenged by PCV2. The results showed recombinant phage inserted with the PCV2 capsid gene was successfully constructed. Western blot assay showed that PCV2 capsid was expressed on the surface of phage T7-Cap. Specific anti-PCV2 antibody induced production in the pigs inoculated with T7-Cap, and the PCV2 virus could be cleared from the T7-Cap immunized pigs after they were challenged by PCV2. These findings indicate T7-Cap may be a suitable alternative candidate vaccine of PCVD because it can easily be produced in large scales, cheap and cost-effective.