Novel Detection of Acrylamide by Electrochemiluminescence Sensor and Optical Imaging Analysis

摘要

Acrylamide, a carcinogen with debilitating effects on human health, was detected byelectrochemiluminescence (ECL) measurement using Ru(bpy) 3 2+ as the luminophore. Under theoptimum conditions, Pt electrode as the working electrode showed a superior linear detection in therange 5 μM–10 mM, with a detection limit of 1.2 μM (S/N = 3). Moreover, a remote wireless camerawas employed to collect the digital images of the ECL emission. These images were converted fromRGB (Red, Green, Blue) model to HSV (Hue, Saturation, Value) model using the MATLAB software.It was found that Value (V) in the HSV model had a linear relationship with the logarithm of theacrylamide concentration, suggesting that acrylamide could be detected in levels of 5 μM using opticalimaging. The ECL method for detecting acrylamide was proved to be simple and convenient. This isexpected to lead to the development of a novel acrylamide detection method for food safety and otherfields.