?Vanadium pentoxide and tungsten oxide as substrates for enzyme immobilization in an EGFET-biosensor

摘要

V2O5 and WO3 were obtained via sol-gel process as candidates to application in biosensors. X-raydiffractograms indicated the presence of lamellar structures, d = 1.17 nm and d = 0.69 nm,corresponding to V2O5.1.8H2O and WO3.2H2O, respectively. SEM images of V2O5 in xerogel form(comment 2) revealed a vanadium pentoxide xerogel ribbons with one dimensional stacking .Diffractogramms corresponding to WO3 in xerogel form (comment 2) revealed a delamination process,which resulted in an irregular stack of rounded platelets. Investigation of the electronic andelectrochemical properties of the samples was conducted using cyclic voltammetry (CV) and extendedgate field effect transistors (EGFET). Glucose oxidase (GOx) was further immobilized to act as thesensing component in a biosensor. CV and EGFET were also used to confirm immobilization of GOxon the oxide surfaces, indicating that the materials are promising candidates for application asdisposable biosensors in the future.