A Chemometrical Analysis of Voltammetric Data for Simultaneous Determination of Phenobarbital Sodium and Paracetamol Obtained at a Gold Electrode

摘要

The electrochemical behavior of phenobarbital sodium (PBS), paracetamol (PCM) and their binarymixtures was investigated using cyclic voltammetry (CV) and square wave voltammetry (SWV) at abare gold electrode in a 0.05 M bicarbonate solution. A calibration curve of PBS obtained by SWV hadtwo linear ranges, from 1.0 to 3.0 M and from 5.0 to 35.0 M with a limit of quantification (LOQ) of0.62 M and a limit of detection (LOD) of 0.19 M, while a calibration curve of PCM was determinedwithin the range from 10.0 to 50.0 M with a LOQ of 8.53 M and a LOD of 2.56 M. Both drugsunderwent oxidation by irreversible, diffusion controlled process. The SW voltammograms of the drugmixtures produced complex, overlapping profiles and a chemometric method was applied for theirdecomposition. Two different artificial neural network (ANN) architectures, namely back-propagationneural network (BPNN) and general regression neural network (GRNN) were employed for thesimultaneous prediction of the concentrations of the drugs in a synthetic sample. The BPNN model hadhigher accuracy (mean absolute percentage error values were in the range of 3.6.4%) and thus it canbe used for the simultaneous determination of PBS and PCM.