A Glucose Biosensor Based on Glucose Oxidase enzyme and ZnO Nanoparticles Modified Carbon Paste Electrode

摘要

In this study, modified carbon paste electrode (CPE) with zinc oxide (ZnO) nanoparticles used fordesigning a glucose oxidase enzymatic biosensor to investigation of its electrochemical behavior, anddetermination of glucose concentration. The size and morphology of synthesized ZnO metal oxidenanoparticles evaluated via chemistry technique like scanning electron microscopy (SEM) and X-raydiffraction (XRD). A pair of clear and stable peaks in PBS ( 0.1 M, pH = 7) obtained for the directtransfer of electrons from GOD to modified electrode with -340 mV and -385 mV respectively asoxidation and reduction potentials. The enzymatic biosensor exhibited a linear response to the -D (+)glucose cancentration range from 40 M to 380 M with limit of detection equal to 8 M in a signal tonoise ratio of 3. Here, the designed GOD/ZnO/CPE electrochemical biosensor showed high stabilityand efficiency for glucose sensing.