Study of Functional Qualities of Different Types of Tailored Liposomes with Encapsulated Doxorubicin using Electrochemical and Optical Methods

摘要

This study points to changes in the properties of liposomes and its functional qualities in task of releaseof encapsulated drug doxorubicin according to the content of cholesterol in the phospholipid bilayer.The influence of sodium dodecyl sulphate (SDS) addition to liposomal variants was also evaluated too.Three variants of liposomes differing in various concentrations of cholesterol were assessed. Firstly,we focused on the toxicity of all liposomal variants to Staphyloccocus aureus and it was found that thecontent of cholesterol increases the IC50 values of encapsulated doxorubicin in liposome with higherconcentration of cholesterol than in liposome without cholesterol even four times. Further, the newapproach to comparing the influence of various liposomes on detection of encapsulated doxorubicinusing the electrochemical detection with construction of differential hydrodynamic voltammogramswas used. Finally, the fluorescence detection was used to confirm the release of encapsulateddoxorubicin from liposomes after the addition of detergent SDS. In this work we demonstrated thesuitability of number of methods including electrochemistry for studying of effect of substances suchsubstance as SDS and cholesterol on the lipid functional qualities.