Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

摘要

Taurine exhibits many positive effects on organism, such as induction of important protectivebiochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurineconsumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and braintissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration oftaurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After-1 optimization, the limit of detection of GSH in brain and liver tissue was established as 40 nmol.g of-1 protein and 90 nmol.g of protein, respectively. That is sufficient for determination of theconcentration alterations of GSH in studied tissues. The consumption of taurine increased content of-1 GSH determined in the brain and liver (increased by approximately 0.8 mmol.g of protein incomparison with control). The increase of concentration of taurine occurred only in the brain, by cca-1 50 g.g of tissue, regardless the applied concentration of taurine. The applied concentration of taurinehad no effect on concentration of metallothionein in any of the examined tissue.