Electrochemical Detection of Single Nucleotide Polymorphism in Short DNA Sequences Related To Cattle Fatty Acid Binding Protein 4 Gene

摘要

Electrochemical nucleic acid biosensor for detection of single nucleotide polymorphism (SNP) in Fattyacid binding protein 4 (FABP4) has been developed by immobilization of single stranded thiolatedprobe DNA (ss-DNA-SH) onto gold surfaces (ss-DNA-SH/Au) and its hybridization with wild andmutant complementary sequences related to Korean cattle FABP4 gene in the presence methyleneblue. The immobilization of ss-DNA-SH onto gold surfaces was characterized by atomic forcemicroscopy (AFM), Fourier transforms infrared microscopy (FT-IR), cyclic voltammetric (CV) andimpendence techniques. The SNP in Korean cattle FABP4 gene was detected by measuring themethylene blue accumulation on ss-DNA-SH/Au electrode hybridized with wild (wild-ds-DNA- SH/Au) and mutant (mutant-ds-DNA-SH/Au) complementary sequences using CV technique. The-1 sensitivities of wild-ds-DNA-SH/Au and mutant-ds-DNA-SH/Au electrodes are 0.0164 Ag L-1 with 0.998 regression coefficient (R) and 0.0122 Ag L with 0.993 R, respectively. The ssDNA- SH/Au electrode can detect the wild and mutant type complementary targets related to FABP4 gene in-1 the range of 5-10 ng L .