Globularifolin exhibits potentanticancer activity on A549 human lungcancer cell line via induction of mitochondrialapoptosis, cell cycle arrest and NF-kB inhibition

摘要

Globularifolin is an iridoid glucoside and has only been isolated from some plant species of plantaginaceae.Iridoids glucosides have been reported to exhibit tremendous pharmacological potential. However, the anticanceractivity of the rare acylated iridoid glucoside globularifolin has not been reported so far. In the present studywe determined its anticancer potential against human lung cancer A549 cancer cell line. The results showed thatglobularifolin has an IC50 value of 12.5 μM human lung cancer cells and inhibited the colony formation potential ina dose-dependent manner. The molecule exerted its anticancer activity through induction of apoptosis by regulatingBcl-2/Bax signaling pathway. Additionally, it caused cell cycle arrest of human lung cancer A549 cancer cells atG2/M phase. Globularifolin was also found to cause reduction in the mitochondrial membrane potential in a dosedependent manner. Additionally, globularifolin effectively inhibited NF-kB almost by 50% at 25 μM concentrationat 24 h incubation. We conclude that globularifolin exerts its anticancer activity through induction of mitochondrialapoptosis, G2/M cycle arrest and NF-kB inhibition indicating it may prove as a lead molecule treatment of lungcancer.