Bioinformatics analysis of growth differentiation factor-9 and bone morphogenetic protein-15 similarity in sheep, goat and cattle

摘要

Litter size is one of the important economic traits in farm mamales. Many genetic and management factors affect on litter size. Growth differentiation factor - 9 (GDF - 9) and Bone morphogenetic protein - 15 (BMP - 15) are two genetic factors affecting this trait in sheep, goat, and cattle. In this study, 6 protein sequences of GDF - 9 and BMP - 15 selected from UniProt database and then some bioinformatics analysis were investigated such as multiple alignment, motifs identify, tertiary structure, and homology search. The results were indicated that GDF - 9 and BMP - 15 protein sequences in sheep , goats and cattle are very similar in their about 112 COOH - terminal amino acids and there are two motifs in both proteins. In other words, COOH - terminal of both genes have similar amino acids and function which support multiple alignment results. This similarity have been conserved and is common in TGF - beta family.