METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OXYBENZONE, OCTINOXATE AND AVOBENZONE IN SUNSCREEN LOTION BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

A simple, precise, accurate, selective, and economic reversed-phase RP-HPLC method has been developed for simultaneous analysis of Oxybenzone, Octinoxate and Avobenzone in sun-screen lotion. Chromatographic separation was performed by using UV detector at wavelength of 330nm. The separation was performed at ambient temperature by using C18 stationary phase followed by methanol: phosphate buffer (90:10) pH (3.0) as a mobile phase. The mobile phase flow rate was 1.2ml/min and gradient elution was performed. Total run time was 25min. The method was successfully applied on formulation and there has been no interference of matrix components. The linearity of method was obtained in the range of 18 to 48μg/ml for Oxybenzone, 45 to 120μg/ml for Octinoxate and 12 to 32μg/ml for Avobenzone. Recovery results obtained for all three components were within the range. All validation parameters were done in accordance with International Conference on Harmonization (ICH). The validation results and results from statistical analysis of the data, demonstrated the method were reliable.