COLUMN CHROMATOGRAPHY FOR ISOLATION OF ANDROGRAPHOLIDE FROM IN VITRO GROWN MEDICINAL PLANT-ANDROGRAPHIS PANICULATA WALL. EX NEES

摘要

Andrographis paniculatawhich is commonly known as Kalmegh is an importantmedicinal plant. The Methanol extract of in vitrogrown A. paniculatawas used for columnchromatography to isolate its active ingredient- Andrographolide. The melting point and UV detectionwave length of the colourless crystal recovered after column chromatography were recorded as 235oC and220nm respectively. Comparison of recorded UV spectral data, characteristic IR spectra and melting pointof isolated constituents with previous literatures indicates that the isolated crystal is the andrographolideof A. paniculata