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首页> 外文期刊>Annals of laboratory medicine. >Comparative Analysis of Clinical Samples Showing Weak Serum Reaction on AutoVue System Causing ABO Blood Typing Discrepancies
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Comparative Analysis of Clinical Samples Showing Weak Serum Reaction on AutoVue System Causing ABO Blood Typing Discrepancies

机译:导致ABO血型差异的AutoVue系统血清反应弱的临床样本比较分析

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Background: ABO blood typing in pre-transfusion testing is a major component of the high workload in blood banks that therefore requires automation. We often experienced discrepant results from an automated system, especially weak serum reactions. We evaluated the discrepant results by the reference manual method to confirm ABO blood typing. Methods: In total, 13,113 blood samples were tested with the AutoVue system; all samples were run in parallel with the reference manual method according to the laboratory protocol. Results: The AutoVue system confirmed ABO blood typing of 12,816 samples (97.7%), and these results were concordant with those of the manual method. The remaining 297 samples (2.3%) showed discrepant results in the AutoVue system and were confirmed by the manual method. The discrepant results involved weak serum reactions (Conclusions: ABO blood tying of 97.7% samples could be confirmed by the AutoVue system and a small proportion (2.3%) needed to be re-evaluated by the manual method. Samples with a 2+ reaction grade in serum typing do not need to be evaluated manually, while those with ≤1+ reaction grade do.
机译:背景:输血前测试中的ABO血型检查是血库高工作量的主要组成部分,因此需要自动化。我们经常会遇到自动化系统产生的差异结果,尤其是血清反应较弱的情况。我们通过参考手册方法评估了差异结果,以确认ABO血型。方法:总共使用AutoVue系统测试了13,113份血液样本;所有样品均按照实验室规程与参考手册方法平行运行。结果:AutoVue系统确认了12,816个样本的ABO血型(97.7%),这些结果与手动方法一致。剩余的297个样本(2.3%)在AutoVue系统中显示出不同的结果,并通过手动方法进行了确认。差异的结果涉及血清反应较弱(结论:可以通过AutoVue系统确认97.7%的样品的ABO血型,并且需要通过手动方法重新评估的比例很小(2.3%)。反应等级为2+的样品血清分型的人不需要手动评估,而反应等级≤1+的人则需要手动评估。

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