Microsatellite marker based diversity analysis for submergence tolerance in some Bengal landraces of rice ( Oryza sativa L.)

摘要

Twenty eight rice genotypes from West Bengal and Assam were studied to assess genetic diversity using three SSR markers linked with QTL of submergence tolerance. Among the three markers, only RM-219 generated polymorphic pattern by producing four alleles, of which two were high frequency alleles. The PIC values derived for this marker was 0.325. All the genotypes exhibited multiple allelism with two alleles, except in ‘Panikalas’ where three alleles were detected. Markers RM-464A and RM-265 were unable to distinguish any of the 28 genotypes and therefore not suitable for marker assisted selection in the indica genotypes. Marker RM-219, however, can be used for monitoring introgression of the Sub-1 locus responsible for submergence tolerance. However, this marker did not show any polymorphism between known submergence tolerant genotypes and non-tolerant genotypes, which would prevent the use of RM-219 for selection of segregating populations derived from parental combination.