Electrochemical Detector Using Carbon Fiber Working Electrode for Micro High Performance Liquid Chromatography

摘要

A tubular electrolytic cell of ultra micro volume, made of a single carbon fiber (7μm o.d., 15mm length) as the working electrode material and a fused silica tube (50μm i.d.) as the flow cell part, was designed for use as the electrochemical detector in micro high performance liquid chromatography (micro HPLC). The effective cell volume is about 0.03μl. This detector with carbon fiber was useful for detection of catecholamines and their metabolites. Their detection limits were about 1-2pg and the linear dynamic range was about 103. The detector was successfully applied to urinary catecholamine analysis by micro HPLC and to rapid detection of electroactive species in fast micro HPLC.