Direct Electrochemical Behavior of Cytochrome c on DNA-modified Glassy Carbon Electrode and Its Application to Cytochrome c Biosensor

摘要

DNA was immobilized on glassy carbon electrodes to fabricate DNA-modified electrodes. The direct electron transfer of horse heart cytochrome c on DNA-modified glassy carbon electrode was achieved. A pair of well-defined redox peaks of cytochrome c appeared at Epc = -0.017 V and Epa = 0.009 V (vs. Ag/AgCl) in 10 mM phosphate buffer solution (pH 7.0) at a scan rate of 50 mV/s. The electron transfer coefficient (α) and the standard rate constant of the surface reaction (Ks) of cytochrome c on DNA-modified electrodes could be estimated to be 0.87 and 34.52 s-1, respectively. The DNA-modified glassy carbon electrode could be applied to detect cytochrome c by means of differential pulse voltammetry (DPV). The cathodic peak current was proportional to the quantity of cytochrome c in the range of 4.0 × 10-6 M to 1.2 × 10-5 M. The correlation coefficient is 0.996, and with the detection limit was 1.0 × 10-6 M (three times the ratio of signal to noise, S/N = 3).