Effects of aqueous extract of leaves of Parkia biglobosa on markers of cellular immunity in rabbit

摘要

The leaves of Parkia biglobosa (Pb) are traditionally used in the treatment of high blood pressure and zoster in C?te d'Ivoire. Our study aims at determining the effect of aqueous extract of leaves of Parkia biglobosa on markers of cellular immunity. Two sets of five (5) batches of six (6) rabbits were formed. The first series each received a single dose of Pb (25, 50, 75 and 100 mg/kg of body weight or BW) and NaCl 0.9%. The second group received Methylprednisolone (15 mg / kg of BW) or MP15 and mixtures of MP15 and Pb (50,75 and 100 mg/kg of BW), and MP15 and Isoprinosine (50mg/kg BW) or Ip50. The rabbit whole blood was collected, from the marginal vein of rabbit ear, in EDTA tubes on the first day before injection (Do), then the 3rd, 9th, 15th and 21th days for the determination of CD4+ count by flow cytometry and the total lymphocyte counts by hemogram. Results showed that single doses of Pb (25, 50, 75 and 100mg/kg BW) induced in the 3rd and 9th days, a significant increase of total lymphocytes and TCD4+ count compared with NaCl 0, 9%. The dose of Pb75 induced the highest count of lymphocytes and CD4+ (P<0.0001). However, in the presence of methylprednisolone, the same dose of Pb (50, 75 and 100 mg/kg BW) did not induce a change in the count of immune cells compared to control (NaCl 0.9%) in contrast to Ip. The results suggest that the aqueous extract of leaves of Parkia biglobosa stimulate the production of total lymphocytes and TCD4+.