Pollen Morphology and Pollen Fertility Estimation of Three Medicinal Plant Species of Hypericum L. from Kaghan Valley, Northern Pakistan

摘要

Pollen morphology & pollen fertility estimation of three medicinal species belonging to genus style="font-family:Verdana;">Hypericum style="font-family:Verdana;">L. from flora of Kaghan valley, Northern Pakistan was investigated by light and scanning electron microscopy for the first time. The species are style="font-family:Verdana;">Hypericum elodeoides style="font-family:Verdana;">Choisy in, style="font-family:Verdana;">Hypericum oblongifolium style="font-family:Verdana;">Choisy, and style="font-family:Verdana;">Hypericum perforatum style="font-family:Verdana;"> L. Pollen morphological characteristics with considerable variation are provided for these taxa. Among the studied taxa, the pollen grains were style="font-family:Verdana;">3-4 colporate type and the largest pollen size was found in style="font-family:Verdana;">H. oblongifolium style="font-family:Verdana;"> in polar view 23.0 μm and in equatorial view was 25.35 μm. The smallest pollen was of style="font-family:Verdana;">H. elodeoides style="font-family:Verdana;"> in polar view which was 19.91 μm and in equatorial view was observed 12.53 μm in style="font-family:Verdana;">H. perforatum style="font-family:Verdana;">. The shape of the all pollen grain was circular to semi-angular in polar view, however in equatorial view the shape of the grains was oblate-spheroidal, in style="font-family:Verdana;">H. elodeoides style="font-family:Verdana;">, and style="font-family:Verdana;">H. oblongifolium style="font-family:Verdana;"> while prolate in style="font-family:Verdana;">H. perforatum. style="font-family:Verdana;">Exine sculpturing was observed by scanning electron microscopy which showed foveolate sculpturing in style="font-family:Verdana;">H. elodeoides style="font-family:Verdana;">, reticulate in style="font-family:Verdana;">H. oblongifolium style="font-family:Verdana;"> and scabrate in style="font-family:Verdana;">H. perforatum. style="font-family:Verdana;"> Pollen fertility estimation ranged from 82.66% to 90.90% which shows that flora of selected species is well established.