Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (Phaseolus vulgaris L.) under in Vitro Conditions by Scanning Electronic Microscopy

摘要

Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes development in four Costa Rican bean varieties (Brunca, Huetar, Guaymi and Bribri) cultivated on MS media with or without 5 mg·L–1 de N6-benzylaminopurine (BAP) by scanning electron microscopy (SEM) was developed in the present work. Micrographs showed similarities and differences in the ultrastructure of the apical dome, epidermal surface, stomata and different types of trichomes in the varieties cultivated on organogenesis media. Genotypes with advantageous morphological characteristics for genetic transformation, in particular an exposed apical dome, were identified. This work will contribute to the optimization of the in vitro regeneration of four common bean varieties.