Nicotiana tabacum style='font-family:Verdana;font-size:12px;'> are helpful to confirm the location of genes on specific chromosomes. In'/> Development of a Method to Produce Chromosome Lacking Lines (CLLs) in Nicotiana tabacum L. “Red Russian”
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Development of a Method to Produce Chromosome Lacking Lines (CLLs) in Nicotiana tabacum L. “Red Russian”

机译:烟草中产生染色体缺失系(CELL)的方法的开发。 “红色俄罗斯人”

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Monosomic lines of style="font-family:Verdana;font-size:12px;">Nicotiana tabacum style="font-family:Verdana;font-size:12px;"> are helpful to confirm the location of genes on specific chromosomes. In the cross style="font-family:Verdana;font-size:12px;">N. nudicaulis style="font-family:Verdana;font-size:12px;"> and style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;">, hybrid seedlings express lethal symptoms, which are controlled by the S subgenome of style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;">. style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;">To identify the responsible chromosome, we style="font-family:Verdana;">needed to style="font-family:Verdana;">produce style="font-family:Verdana;">chromosome lacking lines style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;font-size:12px;"> (CLLs) of style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;"> L. style="font-family:Verdana;">“ style="font-family:Verdana;">Red Russian style="font-family:Verdana;">” style="font-family:Verdana;">and use style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;">them style="font-size:10pt;font-family:;" "=""> style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;font-size:12px;">to cross with style="font-family:Verdana;font-size:12px;">N. nudicaulis style="font-family:Verdana;font-size:12px;">. style="font-size:10pt;font-family:;" "=""> style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;font-size:12px;">From a cross of ( style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;"> × style="font-family:Verdana;font-size:12px;">N. tomentosiformis style="font-family:Verdana;font-size:12px;">) × style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;">, 380 BC1 individuals were obtained. Using a Haplo-Q style="font-family:Verdana;">line style="font-family:Verdana;"> (a monosomic line lacking the single style="font-family:Verdana;">linkage group 11 style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;font-size:12px;">) and style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;">, we found that style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;font-size:12px;">qPCR is a simple and reliable screening method for CLLs of style="font-family:Verdana;font-size:12px;">N. tabacum style="font-family:Verdana;font-size:12px;">. The marker PT30342 is located on linkage group 11 style="font-family:Verdana;">, and style="font-family:Verdana;"> the -Ct value (Ct Actin style="font-family:Verdana;">- style="font-family:Verdana;"> Ct PT30342) was 2.0 for a disomic line and was 1.097 style="font-size:10pt;font-family:;" "=""> style="font-family:Verdana;">for a Haplo-Q line. By the use of style="font-family:Verdana;">flow cytometry, qPCR and chromosome counting together as a screening method, style="font-family:Verdana;"> we identified 6 CLLs lacking 2 to 6 chromosomes. style="font-size:10pt;font-family:;" "
机译:style =“ font-family:Verdana; font-size:12px;”>烟草(Nicotiana tabacum) style =“ font-family:Verdana; font-size: 12px;“>有助于确定特定染色体上基因的位置。在十字架上 style =“ font-family:Verdana; font-size:12px;”> N。 nudicaulis style =“ font-family:Verdana; font-size:12px;”>和 style =“ font-family:Verdana; font-size :12px;“> N。烟草 style =“ font-family:Verdana; font-size:12px;”>,杂交幼苗表现出致命症状,这些症状由 的S亚基因组控制 style =“ font-family:Verdana; font-size:12px;”> N。 tabacum style =“ font-family:Verdana; font-size:12px;”>。 style =“ font-size:10pt; font-family :;” “ =”“> style =” font-family:Verdana;“>要确定负责的染色体,我们 style =” font-family:Verdana;“>需要 style =“ font-family:Verdana;”>产生 style =“ font-family:Verdana;”>缺少线条的染色体 style =“ font-size:10pt ; font-family :;“” =“”> style =“ font-family:Verdana; font-size:12px;”> style =“ font-family :Verdana; font-size:12px;“> N。 tabacum style =“ font-family:Verdana; font-size:12px;”> L. style =“ font-family:Verdana;” >“ style =“ font-family:Verdana;”>红色俄语 style =“ font-family:Verdana;”>” style =“ font- family:Verdana;“>并使用 style =” font-size:10pt; font-family :;“ “ =”“> style =” font-family:Verdana;“>他们 style =” font-size:10pt; font-family :;“” =“”> < / span> style =“ font-size:10pt; font-family :;” “ =”“> style =” font-family:Verdana; font-size:12px;“>与 style =” font-family:Verdana; font-size:12px ;“> N。nudicaulis style =” font-family:Verdana; font-size:12px;“>。 style =” font-size :10pt; font-family :;“” =“”> style =“ font-size:10pt; font-family :;” “ =”“> style =” font-family:Verdana; font-size:12px;“>从( style =” font-family:Verdana; font-size :12px;“>烟草(N. tabacum) style =” font-family:Verdana; font-size:12px;“>× style =” font- family:Verdana; font-size:12px;“> N。tomentosiformis style =” font-family:Verdana; font-size:12px;“>)× style =“ font-family:Verdana; font-size:12px;”>烟草 style =“ font-family:Verdana; font-size:12px;”> ,使用Haplo-Q style =“ font-family:Verdana;”> line style =“ font-family:Verdana;” >(缺少一条 style =“ font-family:Verdana;”>链接组11 style =“ font-size:10pt; font-family :;”的单体行=“”> style =“ font-family:Verdana; font-size:12px;”>)和 style =“ font-family:Verdana; font-size:12px;”” > N。 tabacum style =“ font-family:Verdana; font-size:12px;”>,我们发现 style =“ font-size:10pt ;字体系列:;” “ =”“> style =” font-family:Verdana; font-size:12px;“> qPCR是用于 style =” font-family“的CLL的一种简单可靠的筛选方法:Verdana; font-size:12px;“>烟草(N. tabacum) style =” font-family:Verdana; font-size:12px;“>。标记PT30342位于链接组上11 style =“ font-family:Verdana;”>和 style =“ font-family:Verdana;”> -Ct值(Ct Actin style =“ font-family:Verdana;”>- style =“ font-family:Verdana;”> Ct PT30342)对于二叉线是2.0,并且是1.097 < span style =“ font-size:10pt; font-family :;”“ =”“> style =” font-family:Verdana;“>用于Haplo-Q行。通过使用 style =“ font-family:Verdana;”>流式细胞术,qPCR和染色体计数作为筛选方法, style =“ font-family:Verdana;” >我们确定了6个CLL缺少2至6条染色体。 style =“ font-size:10pt; font-family :;” ”

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