Development of colloidal gold-based immunochromatographic assay for rapid detection of Alicyclobacillus acidoterrestris in apple juice

摘要

ABSTRACT The colloidal gold quick test card which we developed ?￠????was used for rapid detection of Alicyclobacillus acidoterrestris ( A. acidoterrestris ) in apple juice concentrate. Two antibodies against A. acidoterrestris , obtained from Sprague Dawley (SD) rats and Japan White Rabbits, were adopted to construct this quick test card. The results showed that the colloidal gold quick test card had better specificity, and was more ?￠????rapid and convenient which deserved notice especially (the test could be completed within 5?￠????10 min) and concorded with ELISA method (we previously report) and K medium methods (traditional classical method). This new developed colloidal gold quick test card (we named it TAB quick test card) could be more convenient for apple juice safety testing.