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Detection of contaminant bacteria in eggs for consumption in Medellín and its Metropolitan area

机译:检测麦德林及其都会区食用鸡蛋中的污染物细菌

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Objective. Search for Salmonella and other bacteria in commercial eggs. Methods. Thirty-eight neighborhood grocery stores in the city of Medellin and its Metropolitan área were chosen at random; also from each grocery store 6 eggs were selected randomly, for a total of 228 tested eggs. Four samples from every egg were taken, 912 samples in total, which were divided in groups. Every group of samples was cultured in bacteriological media and PCR for Salmonella was performed. Results. The bacterium isolated from the samples were: Bacillus sp., Pseudomonas sp., Enterobacter sp., Serrana sp., Citrobacter sp., E. coli, Streptococcus viridans, Klebsiella sp., Staphylococcus sp., Aeromonas sp., Sarcinas sp., Acinetobacter sp., E. hermanii, Proteus and Stenotrophomonas maltophilia. All cultures and PCR were negative for Salmonella. Conclusions. According to these results, the isolation of contaminating bacteria, such as Aeromonas sp, E. coli, and S. aureus, in commercial eggs is relevant since some of them are potentially pathogenic to humans, especially children, the immunocompromised and the elderly We can conclude that from June to November 2007, the period during which the samples were taken for this current study, Salmonella was not detected in commercial eggs in the city of Medellin and it's Metropolitan Área.
机译:目的。在商品鸡蛋中搜索沙门氏菌和其他细菌。方法。麦德林市及其大都会区的38个附近杂货店是随机选择的;我们还从每个杂货店随机抽取了6个鸡蛋,总共228个测试鸡蛋。从每个鸡蛋中抽取四个样本,总共912个样本,分为几组。每组样品均在细菌培养基中培养,并进行沙门氏菌PCR。结果。从样品中分离出的细菌是:芽孢杆菌属,假单胞菌属,肠杆菌属,Serrana菌,柠檬酸杆菌属,大肠杆菌,绿链球菌,克雷伯菌属,葡萄球菌属,气单胞菌属,Sarcinas菌种。 ,不动杆菌属,hermanii大肠杆菌,变形杆菌和嗜麦芽单胞菌的嗜麦芽癖。所有培养物和PCR沙门氏菌均为阴性。结论。根据这些结果,在商业鸡蛋中分离污染细菌(如气单胞菌,大肠杆菌和金黄色葡萄球菌)非常重要,因为其中一些可能对人类尤其是儿童,免疫力低下的人和老年人具有致病性。结论是,从2007年6月至11月,即本次研究的样本采集期间,在麦德林市和大都会区的商业鸡蛋中未检出沙门氏菌。

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