Important considerations about in vitro production of equine embryos

摘要

The in vitro embryo production (PIVE) in horses is a technique that generates increases in reproductive efficiency of high genetic and commercial value animais. Despite all the studies that has been made, PIVE is still a technique with limited access and success, mainly due to difficulties to achieve efficient rates of fertilization through conventional in vitro fertilization, as well as by the high needs in technical and economic resources for fertilization using intracytoplasmic sperm injection (ICSI), among other limitations on in vitro embryonic development. In the world there have been evaluated multiple alternatives for improving the PIVE in horses: from the use of different means, fluids, cells and molecules, to the use of alternative methods to improve the rates of fertilization through ICSI with the use of substances such as polyvinylalcohol. This review explores the research progress and future expectations for the application of the PIVE technique in horses.