Molecular identification and evaluation of the probiotic ability of lacticacid bacteria from sow colostrum

摘要

Probiotics have become relevant in animal health, especially because of their ability to replace antibiotic growth promoters. This study assessed the probiotic ability of lactic acid bacteria (LAB) isolated from sow colostrum obtained from pig farms in the southwest of Antioquia, Colombia. Bacteria were identified by molecular methods. Colostrum (1 ml) was collected from 20 sows postpartum. Colostrum (0.1 ml) was plated on Man Rogosa and Sharpe agar (MRS) (Merck), and incubated anaerobically at 37 °C for 48h. Probiotic capacity was determined measuring growth at pH 3,0 and growth on 0.3% w/v ox bile salts. Hemolytic activity, antimicrobial activity against Salmonella typhimurium, and sensitivity to antibiotics commonly used in veterinary were also determined. Molecular identification was made by gen16S Ribosomal analysis method. Of all the strains tested, only BAL 1 and BAL3 showed probiotic ability to grow and withstand an acidic pH (3,0) and 0.3% w/v of ox bile. They had γ-hemolytic activity, were catalase-negative, and inhibited growth of Salmonella typhymurium with halos larger than 11 mm. They were sensitive to amoxicillin, penicillin, chloramphenicol and erythromycin, and were identified as Pediococcus pentosaceus. The existence of Pediococcus pentosaceus in sow colostrum and its probiotic ability was demonstrated.