Measurement of Intracellular Free Calcium Concentration of the Starfish Egg by Means of the Microiniection of Aequorin

摘要

References(20) Cited-By(4) Intracellular free calcium concentration was measured in the starfish egg by the luminescence intensity of aequorin microinjected into the cell. In oocyte with a germinal vesicle, the free calcium concentration was about 1.7 p, M in Asterias amurensis and 0.25 p, M or less in Asterina pectinifera. The free calcium concentration was lower in oocyte after disappearance of the germinal vesicle than in oocyte with a germinal vesicle. The starfish egg may have a mechanism for maintaining intracellular free calcium concentration below about 1.7 p, M.