Combined Effects of Tauroursodeoxycholic Acid and Glutamine on Bacterial Translocation in Obstructive Jaundiced Rats:

摘要

Background: Bacterial Translocation is believed to be an important factor on mortality and morbidity in Obstructive Jaundiced. Aims: We investigated the probable or estimated positive effects of tauroursodeoxycholic acid, which has antibacterial and regulatory effects on intestinal flora, together with glutamine on BT in an experimental obstructive jaundiced rat model. Study Design: Animal experimentation. Methods: Forty adult, male, Sprague Dawley rats were used in this study. Animals were randomised and divided into five groups of eight each: sham (Sh); control (common bile duct ligation, CBDL); and supplementation groups administered tauroursodeoxycholic acid (CBDL+T), glutamine (CBDL+G), or tauroursodeoxycholic acid plus glutamine (CBDL+TG). Blood and liver, spleen, MLN, and ileal samples were taken via laparotomy under sterile conditions for investigation of bacterial translocation and intestinal mucosal integrity and hepatic function tests on the tenth postoperative day. Results: There were statistically significant differences in BT rates in all samples except the spleen of the CBDL+TG group compared with the CBDL group (p=0.041, p=0.026, and p=0.041, respectively). Conclusion: It is essential to protect hepatic functions besides maintaining intestinal mucosal integrity in the active struggle against BT occurring in obstructive jaundice. The positive effect on intestinal mucosal integrity can be increased if glutamine is used with tauroursodeoxycholic acid, which also has hepatoprotective and immunomodulatory features. Turkish Ba?l?k:T?kanma Sar?l???nda Meydana Gelen Bakteriyel Translokasyon üzerine Tauroursodeoksikolik Asit ve Glutaminin Birlikte G?sterdikleri Etki Anahtar Kelimeler:Bakteriyel translokasyon, glutamin, tauroursodeoksikolik asit Ama?: Ba??rsak floras?n? düzenleyici ve antibakteriyel etkisi bulunan tauroursodeoksikolik asit (TUDCA) ile birlikte glutaminin deneysel olarak olu?turulan t?kanma sar?l??? modelinde meydana gelen bakteriyel translokasyon üzerine olas? yada tahmin edilen pozitif etkilerini ara?t?rmay? ama?lad?k. ?al??ma Tasar?m?: Hayvan deneyi Y?ntemler: Bu ?al??mada 40 eri?kin erkek Sprague Dawley s??an kullan?ld?. Hayvanlar randomize edildikten sonra her biri 8 rattan olu?an 5 gruba ayr?ld?. S?ras?yla; Sham (sh) grubu, Kontrol (koledok ligasyonu) grubu, ayr? ayr? taurodeoksikolik asit (TUDCA) ve glutamin verilen gruplar ile TUDCA ve Glutaminin birlikte verildi?i grup. Postoperatif 10. gün laparotomi sonras? karaci?er fonksiyon testlerini, ince barsak mukozal bütünlü?ünü ve bakteriyel translokasyonu ara?t?rmak i?in steril ?artlarda karaci?er, dalak, lenf nodu, ileal doku ve kan ?rnekleri al?nd?. Bulgular: Kontrol grubu ile taurodeoksikolik asit ve glutaminin birlikte verildi?i deney grubu kar??la?t?r?ld???nda dalak d???ndaki kan ve doku ?rneklerinde istatistiksel olarak anlaml? farkl?l?k saptand? (p=0.041, p=0.026 ve p=0.041). Sonu?: T?kanma sar?l???nda ortaya ??kan bakteriyel translokasyona kar?? aktif mücadelede intestinal mukozal bütünlü?ü devam ettirmenin yan?nda karaci?er fonksiyonlar?n?n korunmas? da gerekmektedir. Glutamin, hepatoprotektif ve immünmodülat?r ?zellikleri olan TUDCA ile birlikte kullan?ld???nda intestinal mukozal bütünlük üzerindeki olumlu etkisi artabilir.