Analysis of chromatin fibers in Hela cells with electron tomography

摘要

The presence and folding pattern of chromatin in eukaryotic cells remain elusive and controversial. In this study, we prepared ultra-thin sections of Hela cells with three different fixation and sectioning methods, i.e., chemical fixation, high pressure freezing with freeze substitution, and cryo-ultramicrotomy with SEM-FIB (focused ion beam), and analyzed in vivo architecture of chromatin fibers in Hela nuclei with electron tomography technology. The results suggest that the chromatin fibers in eukaryotic Hela cells are likely organized in an architecture with a diameter of about 30?nm. Keywords Chromatin fiber Hela cell Electron tomography Chemical fixation High-pressure freezing Cryo-ultramicrotomy DualBeam-FIB.