ISSR-based genetic diversity of wild and ex situ conserved population of Hopea chinensis (Merr.) Hand.-Mazz

摘要

The genetic diversity and structure of 4 wild population and 2 ex situ conserved population of Hopeachinensis (Merr.) Hand.-Mazz were analyzed and compared by ISSR marker method. Fifty eight bands wereamplified from total genomic DNA with 10 primers in which there were 51 polymorphic, the percentage ofpolymorphic bands of populations ranged from 53.45 to 79.31, with an average value of 69.82, indicating thepresence of high genetic diversity. However, the percentage of polymorphic band, Nei’s diversity index (He)and Shannon information index (I) of wild populations (86.21, 0.3636 and 0.5232, respectively) were higherthan those of ex situ conservated populations (77.59, 0.3153 and 0.4597, respectively), which meant that exsitu conservations of Hopea chinensis had a narrow genetic diversity. Population structure analysis revealedthat genetic variation of Hopea chinensis was 0.0373, so the genetic differentiation was 3.73% amongpopulations, and 96.27% in populations. Furthermore, the strong gene flow (Nm = 12.8896) would be themain reason of limited population differentiation. It concluded that ex situ population had not yet fullycovered the entire genetic diversity of Hopea chinensis.