Effect of Composite Yuyin Recipe on Cultured Human Epithelial Cell Proliferation in vitro

摘要

Objective: To study the effect of Yuyin recipe (YYR), a Chinese composite recipe, on cultured human epithelial cell proliferation in vitro . Methods: YYR in different concentrations was added separately into the keratinocyte CoLo-16 line, after the latter had been cultured 24 hrs, to be co-cultured for different periods of time (24-72 hrs). A blank control group was set up in the meantime. Effect of YYR with different concentrations and different acting time on keratinocyte proliferation was observed by MTT method. Results: YYR in different concentrations showed significant inhibitory effect on keratinocyte CoLo-16 line in concentration-dependent and acting-time-dependent manner. Conclusion: Inhibition on over proliferation of epithelial cells may be one of the mechanisms of YYR in curing psoriasis.