6,7-dimethyllumazine As A Potential Ligand For Selective Recognition Of Adenine Opposite An Abasic Site In Dna Duplexes

摘要

6,7-Dimethyllumazine more selectively binds to adenine (A) base opposite the abasic site in DNA duplexes (5'-TCC AGX GCA AC-3'/3'-AGG TCN CGT TG-5', X = AP site (Spacer C3), N = A, T, C and G) than the other three nucleobases with a dissociation constant K_d of ca. 1.0 μM; substituted methyl groups enhance the binding affinity to A and the selectivity for A over T, compared to the parent molecule, lumazine.rnSingle nucleotide polymorphisms (SNPs) are extremely important as a genetic marker for the identification of disease genes and detection of genetic mutations. Thus, simple and quick detection of SNPs is a key issue in today's chemical biology, biotechnology, medicine and pharmacogenomics.