Rapid Analysis Of Isotopically Unmodified Amino Acids By High-resolution~(14)n-edited ~1h-~(13)c Correlation Nmr Spectroscopy

摘要

A ~(14)N-edited ~(1)H-~(13)C NMR method is described for structural analysis under high-resolution of biomolecules without any enrichment.rnMost of nuclear magnetic resonance (NMR) structural analyses of biomolecules, such as proteins or nucleic acids, are currently performed in solution and are mainly based on the determination of contacts between the three most abundant atoms in these compounds: hydrogen, carbon and nitrogen. However, some biological samples (e.g. membrane and fibrous proteins) are not amenable to solution NMR techniques owing to insolubility or unfavorable relaxation properties. In this case, multidimensional solid-state NMR (SS-NMR) provides a good alternative and enables efficient characterization of large protein domains.