Cell Adhesive Hydrogels Synthesised By Copolymerisation Of Arg-protected Gly-arg-gly-asp-ser Methacrylate Monomers And Enzymatic Deprotection

摘要

This work reports the synthesis of protected Gly- Arg-Gly-Asp-Ser functionalised hydrogels, which are deprotected (and activated for cell adhesion) by reaction with glutathione-S-transferase.rnFunctionalisation with peptides based on the arginine-glyeine-aspartic acid (Arg-Gly-Asp) sequence is one of a range of key technologies for improving the cell adhesive properties of substrates for use as scaffolds in tissue engineering. Two strategies are of general use for covalent attachment: copolymerisation with a suitable peptide monomer or direct reaction with the peptide onto activated surfaces. However, both of these strategies generally use the unprotected peptide so that it is essential to carefully consider the correct choice of reaction media including pH. Also, the Arg residue can bind metals, which can give rise to complications in controlled radical polymerisations.