Single base interrogation by a fluorescent nucleotide: each of the four DNA bases identified by fluorescence spectroscopy

摘要

Nucleoside C, which contains a rigid nitroxide spin label, is effectively reduced in DNA by sodium sulfide to the corresponding amine, yielding a fluorescent probe (C~f) that can report the identity of its base-pairing partner in duplex DNA.rnSingle nucleotide polymorphisms (SNPs) are single nucleotide gene variations. SNPs in a protein-encoding region can result in a mutant protein and subsequently predispose human beings to common diseases and disorders. Thus, disease diagnosis and identification of targets for drug discovery by SNP detection is an active area of research. Most methods that have been developed for detection of SNPs rely on monitoring hybridization of an oligonucleotide probe to the sequence of interest; the probe binds to the wild-type sequence but not to DNAs containing an SNP.