A novel luciferin-based bright chemiluminescent probe for the detection of reactive oxygen speciesf

摘要

This communication reports the synthesis, chemiluminescence properties, and biological application of KEIO-BODIPY-imidazopyrazine (KBI), a yellow-green chemiluminescent probe for the detection of reactive oxygen species (ROS) generated from living cells.rnChemiluminescence analysis is a highly sensitive, rapid, and extremely useful analysis method, which does not require any excitation light source, because the excitation energy is provided by an oxidation reaction. The features of chemiluminescence analysis are ideally suited to use chemiluminescence probes for the detection of reactive oxygen species (ROS) produced by living cells. On one hand, ROS, such as for example super-oxide radical anions (O_2~- ), hydrogen peroxide (H_2O_2), hydro-xyl radicals (·OH), and peroxynitrite (ONOO~-), play a vital role in living organisms. On the other hand, too high or too low ROS concentrations can cause damage to biological functions, and finally result in ageing or various diseases.