Reengineering of a fluorescent zinc sensor protein yields the first genetically encoded cadmium probe

摘要

Introduction of a (Cys)_4 metal binding site at the dimerization interface of two fluorescent protein domains yields a chelating FRET sensor protein that shows a 2500-fold selectivity for Cd~(2+) over Zn~(2+) by taking advantage of their different ionic radii.