A near-infrared reversible fluorescent probe for peroxynitrite and imaging of redox cycles in living cells

摘要

BzSe-Cy is a small-molecule fluorescent probe containing Se, which can respond reversibly to changes in ONOO~ or reduced ascorbate and exhibit high sensitivity and selectivity for ONOO . Redox homeostasis is critical for proper cellular function. The reduction-oxidation state of the cell is primarily a consequence of the precise balance between the levels of reactive oxygen species (ROS) and reducing equivalents.2 Unregulated production of ROS results in potentially cytotoxic "oxidative stress". Many diseases (e.g. Alzheimer's disease) are linked with ROS damage as a result of an imbalance between radical-generating and radical-scavenging systems.4 Thus, the study of intracellular redox cycles has an important physiological and pathological significance.