An orthogonal photolabile linker for the complete 'on-support' synthesis/fast deprotection/hybridization of RNA

摘要

The preparation of a polystyrene solid support decorated with a photolabile linker is described. The entire post-synthetic processing of RNA can be carried out in the solid phase in a minimum amount of time. The deprotected RNA is available for "on-support" hybridization and photolysis releases siRNA duplexes under mild, neutral conditions.