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首页> 外文期刊>Chemical Communications >Enhanced DNA toehold exchange reaction on a chip surface to discriminate single-base changes
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Enhanced DNA toehold exchange reaction on a chip surface to discriminate single-base changes

机译：芯片表面上增强的DNA鞋头交换反应，可区分单碱基变化

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摘要

In this study, a single-base change discrimination strategy was developed through enhanced DNA toehold exchange reaction on a chip surface. A DNA strand with single-base change (SNPs, insertion or deletion) at an arbitrary position was clearly discriminated with nucleic acid concentrations from 0.8 nM to 1 μM through this method.
机译：在这项研究中，通过增强芯片表面上的DNA脚趾交换反应开发了单碱基变化判别策略。通过这种方法，可以清楚地分辨出在任意位置具有单碱基变化（SNP，插入或缺失）的DNA链，其核酸浓度为0.8 nM至1μM。

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  • 来源
    《Chemical Communications》 |2014年第91期|14171-14174|共4页
  • 作者

    Huaguo Xu; Wei Deng; Fujian Huang; Shiyan Xiao; Gang Liu; Haojun Liang;

  • 作者单位

    CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China;

    CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China;

    CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China;

    CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China;

    National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, 230029, People's Republic of China;

    CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China,Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China;

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