Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells

Aoi Teraoka; Kanako Murakoshi; Kyoko Fukamauchi; Akinobu Z. Suzuki; Soichiro Watanabe; Toshiaki Furuta

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Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells

机译：笼状线性DNA的制备和基于亲和力的纯化，用于在哺乳动物细胞中进行光控基因表达

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A facile and useful method for preparing caged DMAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.

机译：开发了一种简便且有用的制备笼状DMA的方法。该方法包括将具有最小蛋白质表达序列的线性dsDNA与Bio-Bhc-重氮进行笼式反应，并关入笼中的DNA。证明了有效的抑制和光介导的蛋白质表达恢复。

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《Chemical Communications》 |2014年第6期|664-666|共3页
作者
Aoi Teraoka; Kanako Murakoshi; Kyoko Fukamauchi; Akinobu Z. Suzuki; Soichiro Watanabe; Toshiaki Furuta;
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Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;

Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;

Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;

Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;

Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;

Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan;

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1. Rabbit DNase .1. Purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships with other mammalian DNases I and phylogenetic analysis [J] . Yasuda T, Takeshita H, Nakajima T, The Biochemical Journal . 1997,第Pta2期

机译：兔DNase .1。从尿液中纯化，免疫和蛋白化学表征，核苷酸序列，在组织中表达，与其他哺乳动物DNase I的关系以及系统发育分析
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机译：兔DNase I：从尿液中纯化，免疫学和蛋白化学表征，核苷酸序列，在组织中的表达，与其他哺乳动物DNase I的关系以及系统发育分析。
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机译：兔DNase I：从尿液中纯化，免疫和蛋白化学表征，核苷酸序列，在组织中的表达，与其他哺乳动物DNase I的关系以及系统发育分析
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机译：发展HEK293 EBNA细胞中哺乳动物CDNA表达分泌瓶颈的综合定性和定量方法
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机译：一种新颖的，基于DNA的α病毒基因表达系统，用于快速重组蛋白纯化和基于病毒的基因传递至视网膜和视网膜母细胞瘤肿瘤细胞。
6. Rabbit DNase I: purification from urine immunological and proteochemical characterization nucleotide sequence expression in tissues relationships with other mammalian DNases I and phylogenetic analysis. [O] . T Yasuda, H Takeshita, T Nakajima, 1997

机译：兔DNase I：从尿液中纯化免疫和蛋白化学表征核苷酸序列在组织中的表达与其他哺乳动物DNase I的关系以及系统发育分析。
7. Rabbit DNase I: purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships with other mammalian DNases I and phylogenetic analysis. [O] . Yasuda, T, Takeshita, H, Nakajima, T, 1997

机译：兔DNase I：从尿液中纯化，免疫和蛋白化学表征，核苷酸序列，在组织中的表达，与其他哺乳动物DNase I的关系以及系统发育分析。
8. Human Cholinesterase Gene Expression in Mammalian Cells: Proposed Nomenclaturefor Human Butyrylcholinesterase Genetic Variants Identified by DNA Sequencing [R] . Lockridge, O., La Du, B. N., Bartels, C. F., 1991

机译：人类胆碱酯酶基因在哺乳动物细胞中的表达：通过DNa测序鉴定的人丁酰胆碱酯酶遗传变异的拟议命名法

Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells

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