Total synthesis of the macrocyclic cysteine knot microprotein MCoTI-II

摘要

The first total synthesis of MCoTI-II,a cysteine knot microprotein and potent trypsin inhibitor,is described;a synthetic strategy has been developed that combines efficient backbone construction via optimised solid phase peptide synthesis with one-pot 'thia-zip' native chemical ligation and refolding to yield the natural product.