One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

Jenny Aveyard; Maryam Mehrabi; Andrew Cossins; Helen Braven; Robert Wilson

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One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

机译：使用金纳米颗粒和核酸横向流（NALF）装置进行PCR产物的一步可视化检测

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Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.

机译：通过将抗体夹在共价附着于金纳米颗粒（GNP）的报告寡核苷酸和共价附着于硝化纤维色谱条的捕获寡核苷酸之间，用无抗体的侧向流动装置检测特异性PCR产物。

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来源
《Chemical Communications》 |2007年第41期|p.4251-4253|共3页
作者
Jenny Aveyard; Maryam Mehrabi; Andrew Cossins; Helen Braven; Robert Wilson;
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作者单位

Department of Chemistry, Liverpool University, Liverpool, L69 7ZD,UK;

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正文语种 eng
中图分类化学;
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2. Simultaneous Detection of Nucleic Acid and Protein Using Gold Nanoparticles and Lateral Flow Device [J] . Xun MAO, Anant GURUNG, Hui XU, Analytical Sciences . 2014,第6期

机译：使用金纳米粒子和侧向流动装置同时检测核酸和蛋白质
3. Visual detection of Zika virus by isothermal nucleic acid amplification combined with a lateral-flow device [J] . Xuemei Lin, Maoyong Wu, Wenbiao Wang, Analytical methods . 2019,第13期

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4. CHEMILUMINESCENT DETECTION OF NUCLEIC ACIDS BY MEANS OF MAGNETIC NANOPARTICLES AND PCR [C] . Li Zhiyang, He Lei, Li Song, The 6'th International Forum on Post-genome Technologies(6'IFPT)（第六届国际后基因组生命科学技术学术论坛） . 2009

机译：磁性纳米粒子和PCR法化学发光检测核酸
5. Engineering Recombinant Antibodies for Immunosensors: Incorporating Peptide Tags for Gold Nanoparticle Binding and Incorporating the 12F6 Antibody in a Lateral Flow Device for Detection of Uranium in Groundwater [D] . Jairo, Grace A. 2018

机译：用于免疫传感器的工程重组抗体：结合用于金纳米颗粒结合的肽标签和结合在侧向流动装置中的12F6抗体，用于检测地下水中的铀
6. Simultaneous Detection of Nucleic Acid and Protein Using Gold Nanoparticles and Lateral Flow Device [O] . Xun Mao, Anant Gurung, Hui Xu, -1

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7. Rapid Molecular Detection of Multidrug-Resistant Tuberculosis by PCR-Nucleic Acid Lateral Flow Immunoassay. [O] . Hatairat Kamphee, Angkana Chaiprasert, Therdsak Prammananan, 2015

机译：pCR-核酸侧向流动免疫分析快速分子检测多药耐药结核病。

One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

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